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. 2010 Jul;177(1):415–423. doi: 10.2353/ajpath.2010.090863

Figure 5.

Figure 5

MET is amplified and constitutively activated in the metastasis-derived H1993 cell line but not in the paired primary lung tumor–derived H2073 cell line. A: Quantitative PCR for MET copy number. MET is not amplified in H2073 cells but is amplified in H1993 cells. Data represent mean ± SEM. Immunoblot reveals elevated Met expression and Y1234/35 and Y1349 phosphorylation in H1993 cells but not in H2073 cells. B-actin is the loading control. B: Growth of H2073 cells is not inhibited by Met shRNA, whereas H1993 cell growth is inhibited. Data represent mean ± SEM. Immunoblot revealing efficient Met knockdown in H2073 and H1993 cells treated with Met shRNA M3 and less efficient knockdown with Met shRNA M2 and M1. L indicates Luciferase shRNA; 0, no shRNA. C: Migration assay was performed in the indicated cell lines as described in Methods. 0, initial time point; 72, 72 hours later; SU, addition of 2.5 μmol/L SU11274 Met inhibitor. Data represent mean ± SEM. *P < 0.5, ***P < 0.001. Results are representative of three independent experiments.