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. 2010 Jul;177(1):473–480. doi: 10.2353/ajpath.2010.090758

Figure 2.

Figure 2

Chondrocyte-like cells within atherosclerotic plaques of C57BL/6 LDL−/− mice (16 weeks high-fat diet). A: Oil-Red O staining (fat = red, nuclei = purple). B: Masson-Goldner trichrome staining (nuclei = blue-black, cytoplasm = red, collagen = green, muscle tissue = bright red). C: Calcein staining (calcium phosphate = green fluorescence, nuclei = blue fluorescence as produced by the DNA-binding dye Hoechst 33258). D: Collagen type II costaining with Hoechst 33258 and β-galactosidase (LacZ) of chondrocyte-like cells. Collagen type II staining (red immunofluorescence), LacZ staining (green immunofluorescence), and blue fluorescence by Hoechst 33258. L indicates lumen; FC, fibrous cap; NC, necrotic core; TA, tunica adventitia; M, tunica media. Scale bar = 100 μm (A–D). E–G: Higher magnification of the area demarcated in D. E: Green channel; F: red channel; and G: red and green channel. Arrows indicate LacZ+ and collagen type II+ stained chondrocyte-like cells. Scale bar = 50 μm (E and F).