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. Author manuscript; available in PMC: 2010 Jun 29.
Published in final edited form as: Exp Cell Res. 2009 Jan 13;315(7):1247–1259. doi: 10.1016/j.yexcr.2008.12.026

Figure 6.

Figure 6

A Western blot demonstrating the expression of full-length gravin-EGFP and its deletion mutants in AN3 CA cells. Sixty µg total protein was loaded in all lanes except the first lane, which was loaded with 10 µg of ECV304 cell lysate containing endogenous gravin (labeled gravin). The second lane was loaded with a lysate from untransfected AN3 CA cells. Other lanes were loaded with lysates from AN3 CA cells transfected with full-length and mutant gravin-EGFP constructs as labeled. The number of amino acids comprising each fusion protein is marked on the bottom of the blot.