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. Author manuscript; available in PMC: 2010 Jun 29.
Published in final edited form as: Exp Cell Res. 2009 Jan 13;315(7):1247–1259. doi: 10.1016/j.yexcr.2008.12.026

Figure 9.

Figure 9

Fluorescent images showing the role of membrane targeting domains in mediating PMA induced translocation of gravin-EGFP in HEC-1-A cells. Deletion of the polybasic regions (ΔPB1,2,3) did not affect targeting of gravin-EGFP to the juxtanuclear compartment after PMA treatment (B and F). However, deletion of the myristoylation site significantly altered translocation of gravin-EGFP in response to PMA and resulted in targeting of the construct to the cytosol only (C and G). PMA treatment had no effect on the cytosolic distribution of gravin-EGFP lacking the myristoylation site and all three polybasic regions (D and H). Scale bar, 20 µm.