Table 2.
Comparison of several measurements of antibody-based protein–protein interactions by AFM
| Molecular partners | Substrate | AFM tip | Unbinding force (pN) |
|---|---|---|---|
| Human IgG/rat anti-human IgG [present study] | Rat anti-human IgG | Human IgG | 144 ± 11 |
| Human serum albumin (HSA)/anti-HSA [34] | HSA | Anti-HSA | 244 ± 22 |
| Sendai-purple membrane (Sendai-PM)/anti-Sendai antibody [2] | Sendai-PM | Anti-Sendai antibody | 70–170 |
| Angiogenin/anti-angiogenin antibody IgG [35] | Anti-angiogenin antibody IgG | Angiogenin | 1029 ± 63 |
| Spore coat protein CotA/anti-CotA [36] | CotA | Anti-CotA | 55 ± 2 |
| Collagen/collagen antibody [37] | Collagen | Collagen antibody | 90 ± 40 |
| HSA/anti-HSA [38] | Anti-HSA | HSA | 88–94 |