Fig. 4.

Kinetics of angiogenesis and tumour growth in surface transplants. (A) Analysis of angiogenesis and tumour invasion in surface transplants of Mmp13+/+ and Mmp13−/− mice. Immunostaining of CD31-positive endothelial cells (red) and pan-keratin-positive tumour tissue (green), nuclei stained by Hoechst (blue), bar 200 μm; T, tumour; S, stroma. (B) Quantification of vessel density in the tumour tissue of surface transplants of Mmp13+/+ and Mmp13−/− animals, bars: means ± SDs of two transplantation series, four animals each, **P < 0.001.