Fig. 5.
mTORC1 signaling inhibition by RAD001 does not alter PI3K/Akt and mitogen-activated protein kinase (ERK1/2) pathways in the thyroid tumors of TRβPV/PVPten+/− mice. Total protein extracts were prepared from thyroid tumors of TRβPV/PVPten+/− mice receiving the placebo (lanes 1–3) or RAD001 (lanes 3–6), as described in Materials and Methods. (A) Western blot analysis of phosphorylated Akt, total Akt, phosphorylated mTOR, total mTOR, phosphorylated GSK3β, total GSK3β and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as a loading control. The ratios of phosphorylated protein to total protein levels, after quantification of the band intensities for each sample, are indicated. (B) Western blot analysis of phosphorylated ERK1/2, total ERK1/2 and GAPDH as a loading control.