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. Author manuscript; available in PMC: 2010 Jun 29.
Published in final edited form as: J Am Chem Soc. 2008 May 23;130(24):7746–7755. doi: 10.1021/ja710348r

Figure 5.

Figure 5

Western blots used to monitor phosphorylation of Akt1 (at both Ser473 and Thr308) as well as eIF4E (Ser209) after 24 h incubation with 50 μM d-3-deoxy-diC8PI (d-3-d-PI), 50 μM l-3-deoxydiC8PI (l-3-d-PI), 10 μM LY 294002 (LY) or 20 nM wortmannin (WM) or a combination LY 294002 and wortmannin (LY+W). The numbers under each lane represent the fractional phosphorylation in the presence of the indicated compound compared to phosphorylation in the presence of l-3-deoxy-diC8PI, which has no effect on the cells.