. Author manuscript; available in PMC: 2010 Jun 29.

Published in final edited form as: J Am Chem Soc. 2008 May 23;130(24):7746–7755. doi: 10.1021/ja710348r

Table 2.

Recombinant PLCδ1 activity toward diC₈PI and deoxy-diC₈PI lipids.

Substrate^a	Inhibitor (mM)^b	$\frac{cIP}{I - 1 - P}$	Specific Activity^c $(\frac{μ mol}{\min - mg})$	Relative Activity
d-diC₈PI		0.93	3.68	1.00
d-3-deoxy-diC₈PI		0.22	0.039	0.011
d-3,5-dideoxy-diC₈PI		0.00	0.018	0.005
d-3-deoxy-diC₈PI(5)P		3.01	0.022	0.006
d-diC₈PI	d-3-deoxy-diC₈PI (2)	1.99	3.01	0.82
d-diC₈PI	d-3-deoxy-diC₈PI5P (6)	1.29	4.81	1.31
d-diC₈PI	d-3,5-dideoxy-diC₈PI (2)	1.77	3.25	0.88
d-diC₈PI	d-3,5-dideoxy-diC₈PI (6)	0.99	3.93	1.07
d-diC₈PI	l-diC₈PI (2)	1.02	3.08	0.84

Substrates present at 2 mM.

The value in parentheses represents the concentration of inhibitor used in these assays.

Errors in specific activity typically <20%.