Table 4.
Inhibitor |
(mM) |
Specific Activity b (μmol min-1 mg-1) |
Relative Activity |
---|---|---|---|
– | 0.60 | 1.00 | |
d-3-deoxy-diC8PI | 3 | 0.09 | 0.15 |
d-3-deoxy-diC8PI(5)P | 3 | 0.49 | 0.81 |
l-diC8PI | 3 | 0.60 | 1.00 |
l-3-deoxy-diC8PI | 3 | 0.11 | 0.18 |
Assay conditions included 1 mM d-diC8PI and 2 mM ATP as substrates, 5 mM Mg2+, in 50 mM Tris HCl, pH 7.5, with 3 mM of the deoxy-PI analogs.
For several of the samples run in duplicate, the error in determining the specific activity was <10%.