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. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: Evol Dev. 2010 Mar-Apr;12(2):131–143. doi: 10.1111/j.1525-142X.2010.00399.x

Figure 3. Protein expression of Artemia ABD-A cannot be induced in Drosophila embryos unless C-terminal sequences are deleted.

Figure 3

(A) Hatch assays of various Artemia ABD-A truncations. The truncation point for each transgene is shown at the bottom of the panel. Hatch percentages were calculated relative to w1118. Ubiquitous expression of Artemia ABD-A protein showed the expected embryonic lethal phenotype only for truncated proteins Artemia ABD-A CΔ and Artemia ABD-A CΔ2. (B) Drosophila embryos induced to ubiquitously express Artemia abd-A transgenes were tested for the presence of transcript and protein. The top panel shows the negative control of embryos from arm-GAL4 crossed to w1118. Transcripts were detected in embryos expressing both full-length and truncated ABD-A, but protein was only detected for embryos expressing the truncated abd-A transgene.