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. Author manuscript; available in PMC: 2011 May 18.
Published in final edited form as: Dev Cell. 2010 May 18;18(5):737–749. doi: 10.1016/j.devcel.2010.03.017

Figure 5. Sca1 is enriched at the leading edge of chemotaxing cells.

Figure 5

(A) Live imaging of LY294002-treated scaA− cells expressing GFP-Sca1 or wild-type cells expressing soluble GFP and migrating in an exponential gradient of cAMP delivered by a micropipette. *, Position of the micropipette. (B) scaA− cells expressing GFP-Sca1 or wild-type cells expressing either the RasG-GTP reporter (GFP-Raf1RBD) or soluble GFP, and migrating under agar in a cAMP gradient, were imaged by TIRFM. Signal from the soluble GFP had to be intensified in order to be visualized. Arrows mark regions of enriched fluorescence. Scale bars represent 5 µm.