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Confocal laser scanning microscopy (CLSM) images of HeLa cells after 4-h incubation with coumarin-6–loaded PLGA-TPGS nanoparticles at 37.0°C. The cells were stained by DAPI (blue) and the coumarin-6–loaded nanoparticles are green. The cellular uptake was visualized by overlaying images obtained by EGFP filter and DAPI filter: left image from EGFP channel (a); center image from DAPI channel (b); right image from combined EGFP channel and DAPI channel (c)
