Figure 7. Mtb-reactive MAIT cells in the human lung.

Single cell suspensions were prepared from the lung and adjacent LNs with minor modifications [52]. The intracellular cytokine staining assay was performed using magnetic-bead purified CD8⁺ T cells from the lung and LNs as described in Figure 6 legend. Only in the case of donor B were anti-MR1 or IgG2a isotype control antibodies added (2.5 µg/ml).