Table 1. Summary of phenotypic data of the candidate genes implicated in HR-DSBR.
| Gene Name | Ensembl ID | Frequency of HR-DSBR | GFP Levels | Viability | Increased Sensitivity | Gamma-H2AX Assay | |||
| esiRNA 1 | esiRNA 2 | Cisplatin | MMC | IR | |||||
| ACTB | ENSG00000075624 | ↓ | ↓↓ | − | − | − | − | − | − |
| AIP | ENSG00000110711 | ↓ | ↓ | − | − | − | ++ | − | − |
| ALOX15 | ENSG00000161905 | ↓↓ | ↓↓ | − | + | − | ++ | − | − |
| ARHGEF1 | ENSG00000076928 | ↓↓ | ↓↓ | − | + | − | + | − | ↓ 1h, ↓ 6h |
| BCAM | ENSG00000187244 | ↓↓ | ↓↓ | − | + | − | + | − | − |
| BRCA1 | ENSG00000012048 | ↓↓↓ | ↓↓↓ | − | + | + | +++ | + | ↑ 6h |
| C1orf63 | ENSG00000117616 | ↓↓ | ↓↓ | − | − | + | + | + | ↑ 6h |
| CHCHD2 | ENSG00000106153 | ↓↓ | ↓↓ | − | + | − | ++ | − | − |
| DRG2 | ENSG00000108591 | ↓↓ | ↓↓ | − | − | − | +++ | − | − |
| ETFB | ENSG00000105379 | ↓↓ | ↓↓ | − | + | − | ++ | − | − |
| FAM110C | ENSG00000184731 | ↓↓ | ↓ | − | + | − | − | − | − |
| FIZ1 | ENSG00000179943 | ↓↓ | ↓ | − | − | − | + | − | ↓ 6h |
| GAK | ENSG00000178950 | ↓↓ | ↓↓ | − | − | − | + | − | − |
| HNRPA0 | ENSG00000177733 | ↓↓ | ↓↓ | − | − | − | + | − | ↓ 6h |
| IGLON5 | ENSG00000142549 | ↓↓ | ↓↓ | − | − | − | + | − | − |
| KIAA0415 | ENSG00000164917 | ↓↓ | ↓↓ | − | − | − | ++ | − | − |
| NTHL1 | ENSG00000065057 | ↓↓ | ↓↓ | − | − | − | +++ | − | ↑ 6h |
| OSBPL5 | ENSG00000021762 | ↓ | ↓↓ | − | − | + | − | ++ | − |
| PRPF40B | ENSG00000110844 | ↓↓ | ↓↓ | − | − | − | ++ | − | ↑ 6h |
| PSMD4 | ENSG00000159352 | ↓↓ | ↓↓↓ | − | + | − | + | − | − |
| RAD51 | ENSG00000051180 | ↓↓↓ | ↓↓↓ | − | + | +++ | +++ | ++ | ↑ 6h |
| RBBP8 | ENSG00000101773 | ↓↓ | ↓↓ | − | − | − | ++ | + | ↑ 6h |
| RBM42 | ENSG00000126254 | ↓↓ | ↓↓ | − | − | − | ++ | − | − |
| RECQL4 | ENSG00000160957 | ↓↓ | ↓ | − | − | − | ++ | + | − |
| SEMA7A | ENSG00000138623 | ↓↓ | ↓↓ | − | + | − | − | − | − |
| SERPINH1 | ENSG00000149257 | ↓↓ | ↓ | − | + | − | − | − | − |
| SHFM1 | ENSG00000127922 | ↓↓↓ | n.a. | − | − | ++ | +++ | − | − |
| TRMT2A | ENSG00000099899 | ↓↓ | ↓ | − | + | + | − | − | ↑ 6h |
| TSKU | ENSG00000182704 | ↓ | ↓↓ | − | − | − | + | − | − |
| XPC | ENSG00000154767 | ↓↓ | ↓↓ | − | + | − | ++ | − | − |
| ZMYND15 | ENSG00000141497 | ↓ | ↓↓ | − | − | − | +++ | − | − |
| ARCN1 | ENSG00000095139 | ↓ | ↓↓ | − | ++ | n.d | n.d | n.d | n.d |
| CKAP5 | ENSG00000175216 | ↓↓ | ↓↓ | − | ++ | n.d | n.d | n.d | n.d |
| CWC22 | ENSG00000163510 | ↓↓↓ | ↓↓ | − | ++ | n.d | n.d | n.d | n.d |
| DDB1 | ENSG00000167986 | ↓↓ | ↓↓ | − | ++ | n.d | n.d | n.d | n.d |
| E2F1 | ENSG00000101412 | ↓ | ↓↓ | − | ++ | n.d | n.d | n.d | n.d |
| HNRPK | ENSG00000165119 | ↓↓ | ↓↓ | − | ++ | n.d | n.d | n.d | n.d |
| PSMD1 | ENSG00000173692 | ↓↓↓ | ↓↓↓ | − | ++ | n.d | n.d | n.d | n.d |
| PSMD14 | ENSG00000115233 | ↓↓ | ↓↓↓ | − | ++ | n.d | n.d | n.d | n.d |
| SNRNP200 | ENSG00000144028 | ↓↓ | ↓↓↓ | − | ++ | n.d | n.d | n.d | n.d |
| THOC4 | ENSG00000183684 | ↓↓ | ↓↓ | − | ++ | n.d | n.d | n.d | n.d |
| TPX2 | ENSG00000088325 | ↓↓ | ↓↓↓ | − | ++ | n.d | n.d | n.d | n.d |
| VPRBP | ENSG00000145041 | ↓↓↓ | ↓↓ | − | ++ | n.d | n.d | n.d | n.d |
| ZYG11BL | ENSG00000160445 | ↓ | ↓↓ | − | ++ | n.d | n.d | n.d | n.d |
| MKNK2 | ENSG00000099875 | ↓↓ | ↓↓ | + | − | n.d | n.d | n.d | n.d |
| ATXN3 | ENSG00000066427 | ↑↑ | ↑↑ | − | − | n.d | n.d | n.d | n.d |
| C5orf28 | ENSG00000151881 | ↑↑ | ↑↑ | − | − | n.d | n.d | n.d | n.d |
| C6 | ENSG00000039537 | ↑↑ | ↑↑ | − | − | n.d | n.d | n.d | n.d |
| CNGA1 | ENSG00000198515 | ↑↑ | ↑↑ | − | − | n.d | n.d | n.d | n.d |
| CREBBP | ENSG00000005339 | ↑ | ↑↑↑ | − | − | n.d | n.d | n.d | n.d |
| DNAJB4 | ENSG00000162616 | ↑↑ | ↑↑ | − | − | n.d | n.d | n.d | n.d |
| LIG4 | ENSG00000174405 | ↑↑ | ↑↑ | − | − | n.d | n.d | n.d | n.d |
| LYRM7 | ENSG00000186687 | ↑↑ | ↑↑ | − | − | n.d | n.d | n.d | n.d |
| MMRN1 | ENSG00000138722 | ↑↑ | ↑↑ | − | − | n.d | n.d | n.d | n.d |
| PDHX | ENSG00000110435 | ↑↑ | ↑↑ | − | − | n.d | n.d | n.d | n.d |
| SLC39A12 | ENSG00000148482 | ↑ | ↑↑ | − | − | n.d | n.d | n.d | n.d |
| SMCHD1 | ENSG00000101596 | ↑↑↑ | ↑↑ | − | − | n.d | n.d | n.d | n.d |
| SMS | ENSG00000102172 | ↑↑ | ↑↑ | − | − | n.d | n.d | n.d | n.d |
| STAG2 | ENSG00000101972 | ↑↑ | ↑↑ | − | − | n.d | n.d | n.d | n.d |
| USP12 | ENSG00000152484 | ↑↑↑ | ↑↑ | − | − | n.d | n.d | n.d | n.d |
| WRB | ENSG00000182093 | ↑↑ | ↑↑ | − | − | n.d | n.d | n.d | n.d |
| XRCC2 | ENSG00000196584 | ↑↑ | ↑↑ | − | − | n.d | n.d | n.d | n.d |
Calculated z-scores using esiRNA against Rluc as negative controls are shown for two independent esiRNA and marked with arrows. Genes that after knockdown decreased the frequency of HR below z-score −4, −2, or −1.5 are marked with ↓↓↓, ↓↓ or ↓, respectively; genes that increase the frequency of HR over z-score 4, 2, or 1.5 are marked with ↑↑↑, ↑↑, or ↑, respectively; n.a., not available. Genes that decreased GFP levels with a z-score>4 are marked with +. Genes that after knockdown decreased viability below 50% and 25% are marked with ++ and +, respectively. Genes that after knockdown decrease the cell number when treated with cisplatin, MMC, or IR by 40%, 30%, or 10% in comparison to Rluc transfection are marked with +++, ++, and +, respectively; n.d., not done. In the last column arrows indicate ↑ increased and decreased ↓ number of gamma-H2AX positive cells after 1 h or 6 h post-IR for knockdowns statistically different (p<0.05) from Rluc transfections.