Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Ubiquitinylation of newly synthesized polypeptides in the presence of AZC requires Ubr1 and Ubr2. Analysis of ubiquitinylation of newly synthesized polypeptides after a 10-min pulse-labeling in the absence (−) and presence (+) of 50 mM AZC. Polyubiquitinylated polypeptides (poly-Ub) were immunoprecipitated with anti-myc and resolved in a 4–20% denaturing gel before phosphorimaging. Strains used were wild-type (WT), ubr1Δ, ubr2Δ, and ubr1Δ/ubr2Δ double mutants.
