Table 1.
Yeast strains used in this study
| Strain | MAT | Genotype | Source |
|---|---|---|---|
| BY4741 | a | his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 | Open Biosystems |
| S288C | a | his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 | Open Biosystems |
| Tpk2-TAP | a | S288C, TPK2-TAP (HIS3) | Open Biosystems |
| Cmk2-TAP | a | S288C, CMK2-TAP (HIS3) | Open Biosystems |
| Cup9-TAP | a | S288C, CUP9-TAP (HIS3) | Open Biosystems |
| Rim11-TAP | a | S288C, RIM11-TAP (HIS3) | Open Biosystems |
| Kss1-TAP | a | S288C, KSS1-TAP (HIS3) | Open Biosystems |
| NNY1 | a | S288C, TPK2-TAP (HIS3) erg6::kanMX6 | This study |
| NNY2 | a | S288C, TPK2-TAP (HIS3) erg6::natMX4 | This study |
| NNY3 | a | S288C, CMK2-TAP (HIS3) erg6::kanMX6 | This study |
| NNY4 | a | S288C, CUP9-TAP (HIS3), erg6::natMX4 | This study |
| NNY5 | a | S288C, RIM11-TAP (HIS3) erg6::kanMX6 | This study |
| NNY6 | a | S288C, KSS1-TAP (HIS3) erg6::kanMX6 | This study |
| NNY7 | a | BY4741, erg6::natMX4, pTPK2 NT-HA 1.111 (LEU2) | This study |
| NNY8 | a | BY4741, ubr1::kanMX6, erg6::natMX4, pTPK2 NT-HA 1.111 (LEU2) | This study |
| NNY9 | a | BY4741, ubr2::kanMX6, erg6::natMX4, pTPK2 NT-HA 1.111 (LEU2) | This study |
| NNY10 | a | BY4741, ubr1::kanMX6, ubr2::HIS3, erg6::natMX4, pTPK2 NT-HA 1.111 (LEU2) | This study |
| NNY11 | a | S288C, TPK2-TAP (HIS3) cdc37S14A::URA3 erg6::kanMX6 | This study |
| NNY12 | a | BY4741, ubr1Δ::kanMX6, erg6::natMX4, pNTFlagUBR1 (LEU2) | This study |
| JN516 | α | his3-11, 3-15, leu2-3,2-112, ura3-52, trp1-Δ1, lys2, SSA1, ssa2::LEU2 ssa3::TRP1 ssa4::LYS2 | E. Craig |
| ssa1-45 | α | his3-11, 3-15, leu2-3,2-112, ura3-52, trp1-Δ1, lys2, ssa1-45, ssa2::LEU2 ssa3::TRP1 ssa4::LYS2 | E. Craig |
| NNY13 | a | S288C, TPK2-TAP (HIS3) erg6::natMX4 ubr1::kanMX6 | This study |
| NNY14 | a | S288C, RIM11-TAP (HIS3) erg6::natMX4 ubr1::kanMX6 | This study |
| NNY15 | a | S288C, KSS1-TAP (HIS3) erg6::natMX4 ubr1::kanMX6 | This study |
| NNY16 | a | S288C, TPK2-TAP (HIS3) erg6::natMX4 ubr2::kanMX6 | This study |
| NNY17 | a | S288C, RIM11-TAP (HIS3) erg6::natMX4 ubr2::kanMX6 | This study |
| NNY18 | a | S288C, KSS1-TAP (HIS3) erg6::natMX4 ubr2::kanMX6 | This study |
| NNY19 | a | S288C, TPK2-TAP (HIS3) cdc37S14A::URA3 | This study |
| NNY20 | a | S288C, TPK2-TAP (HIS3) cdc37S14A::URA3, ubr1::kanMX6, | This study |
| NNY21 | a | S288C, TPK2-TAP (HIS3) cdc37S14A::URA3, ubr2::kanMX6, | This study |
| NNY22 | a | S288C, TPK2-TAP (HIS3) cdc37S14A::URA3, ubr1::kanMX6, ubr2::natMX4 | This study |
| ubr1Δ | a | BY4741, ubr1::kanMX6 | Open Biosystems |
| ubr2Δ | a | BY4741, ubr2::kanMX6 | Open Biosystems |
| NNY23 | a | BY4741, ubr1::kanMX6, ubr2::HIS3 | This study |
| NNY24 | a | BY4741, cdc37S14A::natMX4 | This study |
| NNY25 | a | BY4741, cdc37S14A::natMX4, ubr1::kanMX6 | This study |
| NNY26 | a | BY4741, cdc37S14A::natMX4, ubr2::kanMX6 | This study |
| NNY27 | a | BY4741, cdc37S14A::natMX4, ubr1::kanMX6, ubr2::HIS3 | This study |
| NNY28 | a | BY4741, pHis-Ste11ΔN K444R (URA3) | This study |
| NNY29 | a | BY4741, cdc37S14A::natMX4, pHis-Ste11ΔN K444R (URA3) | This study |
| NNY30 | a | BY4741, cdc37S14A::natMX4, ubr1::kanMX6, pHis-Ste11ΔN K444R (URA3) | This study |
| NNY31 | a | BY4741, cdc37S14A::natMX4, ubr2::kanMX6, pHis-Ste11ΔN K444R (URA3) | This study |
| NNY32 | a | BY4741, cdc37S14A::natMX4, ubr1::kanMX6, ubr2:HIS3, pHis-Ste11ΔN K444R (URA3) | This study |
| NNY33 | a | BY4741, erg6::natMX4 pRS313 TPK2 NT-HA (HIS3) | This study |
| NNY34 | a | BY4741, ubr1::kanMX6, erg6::natMX4 pRS313 TPK2 NT-HA (HIS3) | This study |
| NNY35 | a | BY4741, rad6::kanMX6, erg6::natMX4 pRS313 TPK2 NT-HA (HIS3) | This study |
| NNY36 | a | BY4741, ubc4::kanMX6, erg6::natMX4 pRS313 TPK2 NT-HA (HIS3) | This study |
| NNY37 | a | BY4741, ubc5::kanMX6, erg6::natMX4 pRS313 TPK2 NT-HA (HIS3) | This study |
| NNY38 | a | BY4741, ate1::kanMX6, erg6::natMX4 pRS313 TPK2 NT-HA (HIS3) | This study |
| nta1Δ | a | BY4741, nta1::kanMX6 | Open Biosystems |
| NNY39 | a | BY4741 cdc37S14A::natMX4, nta1::kanMX6 | This study |
| ate1Δ | a | BY4741, ate1::kanMX6 | Open Biosystems |
| NNY40 | a | BY4741 cdc37S14A::natMX4, ate1::kanMX6 | This study |
| rad6Δ | a | BY4741, rad6::kanMX6 | Open Biosystems |
| NNY41 | a | BY4741, cdc37S14A::natMX4, rad6::kanMX6 | This study |
| ubc4Δ | a | BY4741, ubc4::kanMX6 | Open Biosystems |
| NNY42 | a | BY4741, cdc37S14A ::natMX4, ubc4::kanMX6 | This study |
| ubc5Δ | a | BY4741, ubc5::kanMX6 | Open Biosystems |
| NNY43 | a | BY4741, cdc37S14A::natMX4, ubc5::kanMX6 | This study |
| NNY44 | a | BY4741, pRS425 CUP1.mycUB (LEU2) | This study |
| NNY45 | a | BY4741, ubr1::kanMX6, pRS425 CUP1.mycUB (LEU2) | This study |
| NNY46 | a | BY4741, ubr2::kanMX6, pRS425 CUP1.mycUB (LEU2) | This study |
| NNY47 | a | BY4741, ubr1::kanMX6, ubr2::HIS3, pRS425 CUP1.mycUB (LEU2) | This study |
| NNY48 | α | JN516, pRS425 CUP1.mycUB (LEU2) | This study |
| NNY49 | α | ssa1-45, pRS425 CUP1.mycUB (LEU2) | This study |
| NNY50 | a | BY4741, pRS315 CPY* (LEU2) | This study |
| NNY51 | a | BY4741, ubr1::kanMX6, pRS315 CPY* (LEU2) | This study |
| NNY52 | a | BY4741, ubr2::kanMX6, pRS315 CPY* (LEU2) | This study |
| NNY53 | a | BY4741, erg6::natMX4, pHis-Ste11ΔN K444R (URA3), pRS425 CUP1.mycUB (LEU2) | This study |
| NNY54 | a | BY4741, ubr1::kanMX6, ubr2::HIS3 erg6::natMX4, pHis-Ste11ΔN K444R (URA3), pRS425 CUP1.mycUB (LEU2) | This study |
| NNY55 | a | BY4741, san1::kanMX6, erg6::natMX4, pHis-Ste11ΔN K444R (URA3) | This study |
| NNY56 | a | BY4741, erg6::natMX4, pGAL-Arg-β-Gal (URA3) | This study |
a Huntsville, AL.