Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Jul 1;21(13):2128–2137. doi: 10.1091/mbc.E10-03-0200

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2010 by The American Society for Cell Biology

PMC Copyright notice

Figure 6. — dHDAC6 positively regulates the accumulation of insoluble α-synuclein. (A) Immunoblots detecting α-synuclein from the fractions (see Materials and Methods for details of fractionation) of Tris-soluble supernatant (S) and Sarkosyl-insoluble/urea-soluble pellets (P). The flies were 1 d old, 10 d old, and 20 d old, respectively. (B) Western blot of α-synuclein from the heads of 1-d-old flies that express α-synuclein under the control of TH-GAL4 in the backgrounds of wild-type, dHDAC6^KO, and dHDAC6 overexpression, as indicated on the top. The membrane was first probed with α-synuclein antibody and then stripped before immunoblotted with anti-actin that serves as a loading control. Genotypes: α-syn flies are w; TH-GAL4/UAS-α-synuclein. dHDAC6^KO; α-syn flies are w, dHDAC6^KO; TH-GAL4/UAS-α-synuclein. α-syn; dHDAC6 flies are w; UAS-dHDAC6/+; TH-GAL4, UAS-α-synuclein/+.