Figure 1.

Fusel alcohols differentially inhibit translation initiation in butanol-sensitive (BUT^S) and -resistant (BUT^R) strains. (A) Polysome traces from yMK23 (BUT^R) and yMK36 (BUT^S) strains grown in YPD and incubated with added 1-butanol at the concentrations indicated for 10 min at 30°C. Polysomes were analyzed as described in Materials and Methods. The 40S (small ribosomal subunit), 60S (large ribosomal subunit), 80S (monosome), and polysome peaks are labeled. (B) Polysome traces from the yMK23 (BUT^R) and yMK36 (BUT^S) strains as in A, except the yeast were incubated with the indicated concentrations of tert-amyl alcohol (2-methyl 2-butanol), isobutyl alcohol (2-methyl 1-propanol), and isoamyl alcohol (3-methyl 1-butanol) before polysome analysis.