Figure 2.

Parallel mutations within the left-handed parallel β-helix (LbH) domains of eIF2Bε and γ lead to opposite phenotypes after exposure to butanol. (A) Polysome traces from yMK658 (GCD6) or yMK644 (gcd6-1) strains, grown in YPD and treated with butanol at the indicated concentrations for 10 min at 30°C. (B) Polysome traces from yMK974 (GCD1) or yMK976 (GCD1-C483W) strains, grown in selective media and treated with butanol at the indicated concentrations for 10 min at 30°C. Polysome analysis was as described in Materials and Methods.