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. 2010 Jul 1;21(13):2202–2216. doi: 10.1091/mbc.E09-11-0962

Figure 4.

Figure 4.

Specific point mutations but not deletions of the eIF2Bα gene are resistant to butanol treatment. (A) Polysome traces from yMK36 (GCD1-S180) or yMK1262 (GCD1-S180 gcn3Δ) strains, grown in selective media and incubated either in amino acid–free media for 15 min or in media containing butanol at the indicated concentrations for 10 min at 30°C. (B) Polysome traces from gcn3Δ strains bearing various plasmids, yMK1442 with p[GCN3 URA3 CEN], yMK1441 with p[GCN3-R148K URA3 CEN], and yMK1451 with p[GCN3-T41K URA3 CEN]. Strains were grown in selective media and incubated in fresh media or media containing butanol at the indicated concentrations for 10 min at 30°C. Polysome analysis was carried out as described in Materials and Methods. (C) Gradient plate analysis on the above strains showing the resistant profiles of the GCN3 mutant strains relative to the wild type. The gradient plates contain a gradient of butanol up to a maximum of 3% vol/vol, and equal levels of yeast are spotted at every position.