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. 2010 Jul 1;21(13):2241–2256. doi: 10.1091/mbc.E09-11-0930

Figure 2.

Figure 2.

Dynamics of lytic granule movement relative to the MTOC in unconjugated or target cell–conjugated NK cells. (A–C) Time-lapse images of lytic granule movement in conjugates between a YTS GFP-tubulin and a susceptible 721.221 target cell (A), an NK92 GFP-tubulin and a susceptible K562 target cell (B), and a YTS GFP-tubulin and a nonsusceptible K562 target cell (C). In each pair of images, confocal immunofluorescence in the plane of the MTOC is shown on the left and DIC on the right. Green, GFP-tubulin; red, LysoTracker-loaded acidified lysosomes. T = 0 refers to the time that image acquisition began, which was between 1 and 5 min after NK cells were added to the imaging chamber. (D) Quantitative analysis of lytic granule movement relative to the MTOC as measured by mean MTOC to granule distance in individual time points averaged over all measured time points (error bars, ±SD) in conjugates between YTS and 721.221 target cells (n = 10), NK92 and K562 target cells (n = 9), and YTS and K562 target cells (n = 10). All mean distances of lytic granules from the MTOC were significantly different in conjugates compared with unconjugated NK cells (p < 0.05).