Figure 3.

Sec2 mutation results in a reduced number of autophagic bodies, but has no effect on their size. After inactivation, the wild-type (pep4Δ vps4Δ, FRY143), atg1Δ (atg1Δ pep4Δ vps4Δ, HCY76), and sec2-59 (sec2-59 pep4Δ vps4Δ, JGY180) strains were starved for 2 h at 37°C and then analyzed by EM as described in Materials and Methods. (A) Representative images of wild-type, atg1Δ, and sec2-59 strains. Scale bar, 500 nm. (B and C) Number and size of autophagic bodies. Quantification was done as described in Materials and Methods. Error bars, SEM.