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Atg2 is correctly recruited to the PAS in the sec7ts mutant. The sec7ts strain (AFM69-1A) and the WT strain (SEY6210) transformed with the pTS112 vector (GFP-Atg2 under control of the ATG2 authentic promoter) were grown at permissive temperature to an early log phase before rapamycin was added and the cells were either kept at 24°C or transferred to 37°C for 1 h. Fluorescence microscopy was used to image the cells. DIC, differential interference contrast.
