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The assembly of the Atg machinery is not impaired in the sec7ts mutant. (A) WT (FRY341) and (B) sec7ts (AVY046) cells expressing Sec7-dsRed and Atg9-GFP under the control of their authentic promoters were grown at 24°C to an early log phase. Rapamycin was then added and cultures were transferred to the indicated temperatures for 1 h. Alternatively, cells were incubated for 1h at 37°C in the absence of this drug.
