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mph1Δ, shu1Δ, and mms2Δ improve esc2Δ cell growth on MMS-containing media in RAD5, but not rad5-535, background. Wild-type (WT) and mutant cell cultures were diluted and spotted onto YPD plates with or without the indicated concentration of MMS. In A–C, strains are in RAD5 background, and the MMS sensitivity of esc2Δ is suppressed by mph1Δ (A), shu1Δ (B), and mms2Δ (C). In D–E, strains contain rad5-535 as indicated.
