Table 2.
Percent BiFC positive cells in wells transfected with a combination of Vpr and Gag expression plasmids.
| Vpr plasmids | VC-Gag | VN-Gag |
|---|---|---|
| VC-Vprwt | 0 | 31 |
| VN-Vprwt | 27 | 0 |
| VC-VprA30L | 0 | 0 |
| VN-VprA30L | 0 | 0 |
| VC-VprΔ44 | 0 | 0 |
| VN-VprΔ44 | 0 | 0 |
| VC-VprE48A | 0 | 29 |
| VN-VprE48E | 28 | 0 |
| VC-VprL68E | 0 | 0 |
| VN-VprL68E | 0 | 0 |
| VC-VprH71R | 0 | 0 |
| VN-VprH71R | 0 | 0 |
| VC-VprR80A | 0 | 33 |
| VN-VprR80A | 27 | 0 |
293T cells were cotransfected with combinations of VC and VN-chimeric Vpr and Gag expression plasmids as described in methods. Thirty-six hours post transfection cells were fixed and assessed by flow cytometry. BiFC positive (%) cells are presented in the respective combination. Results represent one of three independent experiments with similar results.