Table 2.
Vpr plasmids | VC-Gag | VN-Gag |
---|---|---|
VC-Vprwt | 0 | 31 |
VN-Vprwt | 27 | 0 |
VC-VprA30L | 0 | 0 |
VN-VprA30L | 0 | 0 |
VC-VprΔ44 | 0 | 0 |
VN-VprΔ44 | 0 | 0 |
VC-VprE48A | 0 | 29 |
VN-VprE48E | 28 | 0 |
VC-VprL68E | 0 | 0 |
VN-VprL68E | 0 | 0 |
VC-VprH71R | 0 | 0 |
VN-VprH71R | 0 | 0 |
VC-VprR80A | 0 | 33 |
VN-VprR80A | 27 | 0 |
293T cells were cotransfected with combinations of VC and VN-chimeric Vpr and Gag expression plasmids as described in methods. Thirty-six hours post transfection cells were fixed and assessed by flow cytometry. BiFC positive (%) cells are presented in the respective combination. Results represent one of three independent experiments with similar results.