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. 2010 Jun 29;5(6):e11373. doi: 10.1371/journal.pone.0011373

Figure 5. iPSC Clone Tracking.

Figure 5

Genomic DNA was isolated from teratoma samples and compared with their parent cell lines for TCR β chain rearrangements. Representative data is shown from cell line TiPS V-1. The derivative teratoma harbors the clonal rearrangement of the parent cell line. PCR analysis was conducted using multiplexed primers targeted to conserved regions within the V-J region of the TCR β locus. DNA fragment analysis was performed on an ABI 3730 DNA analyzer. Background ≤1000 RFU.