Table 1. Fission yeast strains, human cell lines, plasmids and HIV-1 viral stocks.

Strains/Plasmids	Genotype and Characters	Source or Reference
S. pombe strains:
SP223	Wild type, h−, ade6-216, leu1-32, ura4-294	[26]
RE078	SP223 with single copy of gfp-vpr integrated at ura4 locus; used for determining nuclear localization of Vpr	[20]
Cut8	h-, leu1, ura4, Δcut8::ura4+	[33]
Mammalian cell lines:
HeLa	A cervical epithelial cell line	ATCC
MAGI-CCR5	A CD4-positive derivative of HeLa cell line; containing an integrated HIV-1 LTR-driven β-galactosidase reporter gene	NIH AIDS Research and Reference Reagents Program
S. pombe Plasmids:
pYZ1N	Fission yeast expression vector with an inducible nmt1 promoter and a leu1 selectable marker	[26]
pYZ3N	Same as pYZ1N but with a 5′ GFP-tag	[26]
pSF173	Fission yeast expression vector with an inducible nmt1 promoter and a 5′ HA-tag, ura4 selectable	ATCC
Mammalian plasmids:
pCDNA3.1	Mammalian expression vector with a CMV promoter; hygromycin-resistant	Invitrogen
pcDNA3.1-HA-Vpr	HA-tagged Vpr on pcDNA3.1	Nathaniel R. Landau
pCDNA3-HA-Kir2.1	HA-tagged Kir2.1., a control plasmid for HA-Vpr	Paul Weilling
pSG5-β1-ZZ	Proteasomal β1 subunit cloned in the pSG5-ZZ plasmid	[38]
pSG5-ZZ	A control plasmid for pSG5-β-ZZ	[38]
HIV-1 viral stocks:
HIV-1Ada Vpr (+)	Packaged virus using the HIV-1 Ada strain with wild type Vpr	[20]
HIV-1Ada Vpr (−)	Packaged virus using the HIV-1 Ada strain with a mutant Vpr	[20]