Figure 1.

a–cRepresentative AFM topographical images of resting T lymphocyte.a3-D image of a resting T lymphocyte;berror-signal mode image ofa, the size of cell is 4.5 μm in diameter and 1.87 μm in height as shown in height profile (i), and cellular pseudopodia at the edge of cell could be clearly seen (b);cnanostructural image.d–hRepresentative AFM images of activated T lymphocytes.d3-D image of an activated T lymphocyte;eerror-signal mode image ofd, the size of cell is 5.469 μm in diameter and 2.34 μm in height as shown in height profile (j). The lamellipodia-like protrusion was also clearly visible (white dotted lineindandarrowine, whose height is 310.1 nm as shown in height profile (k).gAn enlarged error-signal mode image of the square frame inf;hnanostructural image possessing many concaves and the size of the largest concave is 192.2 nm in diameter and 62.15 nm in depth, which is shown by profile (l) that generated along the broken line inh