Fig. 2.

Mutation of the gating glutamate interferes with the physiological role of Gef1 under iron starvation. (A) Growth curves of Δgef1 strains bearing the indicated plasmids in minimal SC medium lacking leucine. n=9 (three independent colonies measured in triplicate in a 96-well plate); error bars indicate s.e. (B) The same experiment as in A with 7.5 μM iron chelator bathophenanthroline sulfonate (BPS) added to the medium. (C) Bar diagram illustrating final OD₆₀₀ after 21 hours of growth for the different constructs. Error bars indicate s.e. and P value for improved growth of Gef1-variant bearing strains in minimal medium is indicated.