Table 1.
Target oligonucleotide sequences
| ssDNA | No. | Sequence (5′-3′) |
|---|---|---|
| 175wd |
|
GGCACCCGCGTCCGCGCCATGGCCATCTAC |
| 175G–A |
1 |
GGCACCCGCGTCCCGCCATGGCCATCTAC |
| 245wd |
|
TGCATGGGCGGCATGAACCGGAGGCCCATC |
| 245G–T |
2 |
TGCATGGGCGCATGAACCGGAGGCCCATC |
| 245G–A |
3 |
TGCATGGGCGCATGAACCGGAGGCCCATC |
| 248wd |
|
GCATGAACCGGAGGCCCATCCTCACCATCA |
| 248G–A |
4 |
GCATGAACCGAGGCCCATCCTCACCATCA |
| 248G–T |
5 |
GCATGAACCGAGGCCCATCCTCACCATCA |
| 248C–T |
6 |
GCATGAACGGAGGCCCATCCTCACCATCA |
| 249wd |
|
GCATGAACCGGAGGCCCATCCTCACCATCA |
| 249G1–T |
7 |
GCATGAACCGGAGCCCATCCTCACCATCA |
| 249G2–T |
8 |
GCATGAACCGGAGCCCATCCTCACCATCA |
| 249G-C |
9 |
GCATGAACCGGAGCCCATCCTCACCATCA |
| 273wd |
|
GAACAGCTTTGAGGTGCGTGTTTGTGCCTG |
| 273G–T |
10 |
GAACAGCTTTGAGGTGCTGTTTGTGCCTG |
| 273G–A |
11 |
GAACAGCTTTGAGGTGCTGTTTGTGCCTG |
| 282wd |
|
TCCTGGGAGAGACCGGCGCACAGAGGAAGA |
| 282C–T | 12 | TCCTGGGAGAGACGGCGCACAGAGGAAGA |
The underline corresponds to the single base mismatch comparing with the wildtype (wd). 12 of the most frequent point mutations in exon 5, 7, and 8 of human p53 gene are included. 248wd and 249wd are the same sequences. The mutant sequences are numbered from 1 to 12