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HPLC confirmation of RGD coupling to the liposomes. (A) Free RGD at 250 μg/mL eluted with a retention time of ~20 minutes; (B) Free RGD at 15 μg/mL also eluted with a retention time of ~20 minutes, and the peak areas of the peaks in A and B demonstrate dose-dependence; (C) The liposome sample modified with the same concentration of 15 μg/mL RGD following the coupling step showed no significant peak for the free RGD around 20 minutes.
