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. Author manuscript; available in PMC: 2010 Jun 30.
Published in final edited form as: Mol Cell. 2000 Apr;5(4):737–744. doi: 10.1016/s1097-2765(00)80252-x

Figure 1.

Figure 1

p48 Confers UV-DDB to Hamster Cells

(A) Deficiency of UV-DDB in hamster cells. UV-DDB was measured in human and hamster peripheral blood lymphocytes and human (HeLa) and hamster (V79) cell lines. Whole-cell extracts were incubated with a 148 bp 32P-labeled DNA probe, which was either left intact (−) or irradiated with 5000 J/m2 of UV (+), and resolved by electrophoretic mobility shift assay (EMSA) (Hwang et al., 1999). B1 and B2 mark the mobility of UV-DDB/DNA complexes. F marks the mobility of free DNA probe. (B) Expression of UV-DDB in cells transfected with p48. Hamster V79 cells were stably transfected with an expression vector lacking a cDNA insert (vec) or containing cDNA encoding p48 or FLAG-p48. Extracts (2 μg) were assessed for UV-DDB by EMSA. Levels of p48, p125, and actin mRNA were measured by Northern blot of total RNA (10 μg per lane), and levels of FLAG-p48 protein were measured by immunoblot with anti-FLAG antibodies (2 μg/ml) (Hwang et al., 1998).