Figure 3.
p48 Expression Does Not Affect UV Survival but Suppresses Mutagenesis
(A) UV survival. Wild-type hamster cells (V79 or AA8), V79 hamster cells transfected with p48 or vector, and hamster cells with mutations in the Cockayne syndrome group B gene (CSB-) or the XP group D gene (XPD-) were exposed to UV from a Westinghouse IL782–30 germicidal lamp at an incident dose of 0.33 J/m2/s. Surviving colonies were counted after 2 weeks. (B) UV-induced mutagenesis. Mutations in the HPRT gene were measured in hamster cells stably transfected with p48, FLAG-p48, or vector. Cells were grown asynchronously (asyn) or synchronized in G1 (syn) by serum starvation, exposed to different doses of UV, and selected for HPRT mutations with 6-thioguanine.
