Table 1.
UV-Induced HPRT Mutations from Hamster Cells Transfected with Vector or p48
| Mutations in Cells Transfected with Vector (1A) |
Mutations in Cells Transfected with p48 (3B4) |
||||||
|---|---|---|---|---|---|---|---|
| Strand of Mutagenic Lesiona |
Nucleotide Position in ORF |
Base Change with Adjacent Sequence in Nontranscribed Strand |
Amino Acid Change |
Strand of Mutagenic Lesiona |
Nucleotide Position in ORF |
Base Change with Adjacent Sequence in Nontranscribed Strand |
Amino Acid Change |
|
|
|
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| − | 62 | ATT (T>A) ATT | L>Stop | − | 114 | TTC (C>T) TCA | P>L |
| − | 71 | GTA (T>A) TCC | I>N | − | 221 | AAT (T>C) CTT | F>S |
| − | 74 | TTC (C>T) TAA | P>L | − | 223 | TTC (T>G) TTG | F>V |
| − | 110 | TTA (T>A) TCC | I>N | − | 245 | ACA (T>A) TAA | I>N |
| − | 145 | AGA (C>T) TTG | L>F | − | 245 | ACA (T>A) TAA | I>N |
| − | 181 | CAT (C>T) ACA | H>Y | − | 274 | AGA (T>G) CCA | S>A |
| − | 194 | CCC (T>G) CTG | L>R | − | 295 | GAT (T>C) TTA | F>L |
| − | 194 | CCC (T>A) CTG | L>H | − | 297 | TTT (T>G) ATC | F>L |
| − | 221 | AAT (T>G) CTT | F>C | − | 347 | AAG (T>G) TAT | V>G |
| − | 223 | TTC (T>C) TTG | P>L | − | 410 | TAA (T>G) TGA | I>S |
| − | 254 | CAC (T>C) GAA | L>P | − | 464/465 | ACC (CC>TT) AAA | P>L |
| − | 296 | ATT (T>G) TAT | F>C | − | 488 | GCT (T>G) GCT | L>W |
| − | 297 | TTT (T>A) ATC | F>L | − | 532 | GAC (T>G) TTG | F>V |
| − | 305 | GAC (T>G) GAA | L>R | − | 542 | GAT (T>A) TGA | F>Y |
| − | 325 | GAT (C>T) AGT | Q>Stop | − | 542/543 | GAT (TT>AA) GAA | F>Stop |
| − | 325 | GAT (C>T) AGT | Q>Stop | 1- | 40 | GAT (G>T) AAC | E>Stop |
| − | 374 | CTT (T>G) AAC | L>Stop | + | 119 | ATG (G>A) AGT | G>E |
| − | 463 | AAC (C>T) CCA | P>S | + | 119 | ATG (G>A) AGT | G>E |
| − | 473 | TGG (T>G) TAA | V>G | + | 139 | ACT (G>A) AAA | E>K |
| − | 487 | AGC (T>G) TGC | L>V | + | 247 | ATT (A>T) AAG | K>Stop |
| − | 490/491 | TTG (CT>TC) GGT | L>S | + | 538 | GTT (G>A) GAT | G>R |
| − | 508 | TCT (C>T) GAA | R>Stop | + | 538 | GTT (G>A) GAT | G>R |
| − | 596 | ACT (T>C) CAG | F>S | + | 538 | GTT (G>A) GAT | G>R |
| + | 124 | GTG (A>T) TTA | I>F | + | 543 | TTT (G>T) AAA | F>Stop |
| + | 229 | GCT (G>T) ACC | D>Y | + | 556 | GAC (A>T) AGT | K>Stop |
| + | 247 | ATT (A>T) AAG | K>Stop | + | 580 | CTT (G>A) ACT | D>N |
| + | 539 | TTG (G>T) ATT | G>V | + | 606 | TTT (G>T) AAT | L>F |
| + | 544 | TTT (G>T) AAA | E>Stop | + | 628 | AGT (G>T) AAA | E>Stop |
| + | 589 | AAT (G>T) AGT | E>Stop | NP | 349 | GTT (A>T) TTG | I>F |
| + | 606 | TTT (G>T) AAT | L>F | NP | 522 | ATA (T>A) AGG | Y>Stop |
| NP | 611 | ATC (A>C) TAT | H>P | NP | 584 | ACT (A>C) TAA | Y>S |
| UD | Del 486–537 | Not defined | Trunc | UD | 246 | CAT (−T) AAA | Trunc |
| UD | Del 323–390 | Not defined | Trunc | ||||
| UD | Del 385–405 | Not defined | Trunc | ||||
| UD | Del 385–402 | Not defined | Trunc | ||||
| UD | Del 533–614 | Not defined | Trunc | ||||
a
The strand of the mutagenic lesion was either the transcribed (+) or nontranscribed (−) strand depending on which strand contained a dipyrimidine sequence at the site of the mutation. NP, no pyrimidine dimer was associated with the mutation. UD, strand with the mutagenic lesion was undetermined. Deletion (Del) in the cDNA led to a truncated (Trunc) open reading frame (ORF), but the mutation in genomic DNA was not defined.