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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: Neurobiol Aging. 2010 Feb 4;32(12):2287–2298. doi: 10.1016/j.neurobiolaging.2010.01.003

Figure 5.

Figure 5

Hirano bodies significantly decrease AICD-induced apoptosis in H4 cells. H4 stable cells stably expressing GFP or CT-GFP were transiently transfected with APPc58-myc DNA (0, 0.5, or 1 μg DNA), and examined at 24 hours for cell viability (A) and caspase 3/7 activation (B). A. Cells were stained with propidium iodide (PI) and Hoechst dye 33342 to assess cell viability. CT-GFP stable cells with Hirano bodies showed a lower percentage of cell death than GFP stable cells (p < 0.001). B. Caspase-3 activity was measured using a fluorescence assay that was normalized to β-galactosidase activity as a control for transfection efficiency of APPc58-myc. Caspase-3 activity represented by relative fluorescence units (RFU) was significantly decreased in CT-GFP stable cells compared to GFP stable cells (p < 0.001). Data shown are mean ± S.E.M. of triplicate measurements, and repeated in three independent experiments. Cell lysates transiently transfected with various concentrations of APPc58-myc DNA were examined by immunoblotting with anti-c-Myc antibodies (bottom panel) to verify that expression of APPc58-myc increased with the amount of DNA transfected.