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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Cell Death Differ. 2009 Aug 28;17(2):268–277. doi: 10.1038/cdd.2009.121

Fig 5. Mechanism of the loss-of-function of cleaved Beclin 1 fragments.

Fig 5

(a)-(b) Beclin 1-GFP (Bec-GFP), N-terminal caspase-cleaved fragment of Beclin 1 (Beclin 1-N)-GFP (Bec-N-GFP), C-terminal caspase-cleaved fragment of Beclin 1 (Beclin 1-C)-GFP (Bec-C-GFP) were transfected into HeLa cells respectively. After 18 hours, cells were fixed. The cells were later viewed under fluorescence microscope (a) and confocal microscope (b).

(c) Myc-Beclin 1 (Myc-Bec), Myc-Beclin 1-N (Myc-Bec-N), Myc-Beclin-C (Myc-Bec-C) were transfected into HeLa cells. After 20 hours, cells were fixed and stained with anti-Myc (9E10). The cells were viewed under confocal microscope.

(d) Vps34/vector, Vsps34/Beclin 1-Flag (Bec-Flag), Vps34/Beclin 1-N-Flag (Bec-N-Flag), Vps34/Beclin 1-C-Flag (Bec-C-Flag) were transfected into HeLa cells respectively. After 20 hours, cells were harvested and lysed. The cell lysates were subjected to anti-Flag antibody immunoprecipitation. The immunoprecipitates were used for SDS-PAGE and western blotting with anti-Vps34 antibody (top panel) and anti-Flag antibody (middle panel) respectively. The total lysates were detected with anti-Vps34 (bottom). Three independent experiments were perfomed with similar results.