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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Cell Death Differ. 2009 Aug 28;17(2):268–277. doi: 10.1038/cdd.2009.121

Fig 6. Bcl-xL rescues the inhibitory effects of Bax on autophagosome formation.

Fig 6

(a) GFP-LC3 was transfected with empty vector, Bax, Bax-Bcl-xL or Bcl-xL into HeLa cells. After 20 hours, cells were fixed and the numbers of GFP puncta per cell were scored. *: P<0.05.

(b) Pictures were taken under fluorescent microcope (100X). Bar: 5um.

(c) HeLa cells were transfected with empty vector, Bax, Bax-Bcl-xL or Bcl-xL in duplicate. After 20 hours, one set of cells were treated with Baf (lanes 5-8). Cells were harvested and blots were probed with anti-LC3, Bcl-xL, Bax and tubulin respectively. Note that endogenous LC3-I gives a very weak signal in HeLa cells compared to LC3-II.

(d) HeLa cells were transfected with empty vector, Bax, Bax-Bcl-xL or Bcl-xL. After 20 hours, cells were harvested and blots were probed with anti-Beclin 1 and tubulin respectively.