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. 2010 Jun 28;189(7):1129–1142. doi: 10.1083/jcb.200912045

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© 2010 Theerthagiri et al.

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Figure 2. — Removal of Nup188–Nup93 enlarges nuclei. (A and C) Western blot analysis of mock- and Nup205–Nup93 (A)- or Nup188–Nup93 (C)-depleted extracts. (B and D) Nuclei were assembled in mock- and Nup205–Nup93 (B)- or Nup188–Nup93 (D)-depleted extracts for 90 min, fixed with 4% PFA and 0.5% glutaraldehyde, and analyzed for chromatin and membrane staining (blue, DAPI; red, DiIC18). (E) Quantitation of the cross-sectional area of mock- and Nup188–Nup93-depleted nuclei of experiments performed as in D. More than 100 randomly chosen chromatin substrates were counted per reaction. The mean of three independent experiments is shown, and error bars represent the total variation. Bars, 20 µm.