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. 2010 Jun 28;189(7):1143–1155. doi: 10.1083/jcb.201001013

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© 2010 Carroll et al.

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Figure 1. — CENP-C binds directly to CENP-A nucleosomes. (A) [³⁵S]methionine-labeled CENP-C was incubated alone (−) or in the presence of α-satellite DNA or nucleosomes (300 nM). The mixtures were resolved on a native gel, and [³⁵S]methionine-labeled CENP-C was detected with a phosphorimager. The position of CENP-C bound to DNA or nucleosomes is indicated. (B) A schematic showing the junctions of the CENP-A (blue) and histone H3 (red) chimeras used in this study. The numbers below the diagram refer to amino acids positions (A.A.) within CENP-A. Helical elements (black boxes, labeled α) and loops (labeled L) found within the histone fold of CENP-A and the region encompassing the CATD are shown above for reference. (C) Wild-type nucleosomes or nucleosomes containing CENP-A/H3 chimeras (500 nM) were incubated with [³⁵S]methionine-labeled CENP-C and resolved on a native gel. [³⁵S]methionine-labeled CENP-C alone (−) was used as a negative control. The position of nucleosome-bound CENP-C is indicated. (D) Clustal W alignment of the C terminus of human histone H3 and CENP-A orthologues from humans (Hs), mice (Mm), chickens (Gg), X. laevis (Xl), C. elegans (CeHCP3), S. pombe (SpCnp1), and Saccharomyces cerevisiae (ScCse4). The identical residues within each protein are highlighted with a black background, whereas conservative amino acid changes are colored gray. The first and last amino acid positions of the residues contained within the alignment are shown on the left and right side of each sequence, respectively. The positions of loop 2 (L2) and helix 3 (α3) within CENP-A are shown above for reference. (E) [³⁵S]methionine-labeled CENP-C was incubated alone (−) or in the presence of the indicated nucleosomes and analyzed as in A. (F) The crystal structure of histone H3 is shown. Amino acids positions analogous to the CENP-A CATD (red ribbon) and residues C-terminal to helix 3 (green dots) are highlighted. Only one of the C-terminal extensions is colored green on the right panel for clarity.