Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Jun 28;189(7):1143–1155. doi: 10.1083/jcb.201001013

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2010 Carroll et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 2. — Identification of a CENP-A recognition module within CENP-C. (A) Full-length (1–943) and various truncation mutants of [³⁵S]methionine-labeled CENP-C were produced in rabbit reticulocyte extracts and resolved on a 12.5% SDS-PAGE gel. The amino acids within CENP-C that are included in each protein are indicated below the gel. The positions of molecular weight markers (kD) are indicated to the left of the gel. (B) Each CENP-C protein was incubated alone (−) or in the presence of 300 nM of CENP-A nucleosomes (+) and resolved on a native gel. (C) CENP-C^426–537 was incubated alone (−) or in the presence of α-satellite DNA or the indicated nucleosome (10 nM) and resolved on a native gel. (D and E) CENP-C^426–537 was incubated alone (−) or in the presence of the indicated nucleosomes (10 nM) and analyzed as above. The specific regions of CENP-A and H3 included in each chimera are schematized in Fig. 1 B.