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. 2010 Jul;136(1):35–46. doi: 10.1085/jgp.200910379

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© 2010 Iovannisci et al.

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Figure 1. — Orientation of epithelium, pH electrode, and titrator burette in Ussing chamber with serosal HEPES-buffered and mucosal unbuffered solutions during pH stat experiments. Solutions were continuously gassed with O₂ (serosally) and N₂ (mucosally) to mix the solutions and prevent air CO₂ from entering.