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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Mol Oral Microbiol. 2010 Apr;25(2):89–101. doi: 10.1111/j.2041-1014.2010.00569.x

Figure 1.

Figure 1

Knockdown of Akt by small interfering RNA (siRNA) in primary gingival epithelial cells. A target-specific Akt antibody was used to confirm the inhibition of Akt expression by Western blotting. A non-target antibody (β-actin) was used to control proper loading and specificity of Akt siRNA. Column 1 is non-target siRNA (control), column 2 is transfection agent alone (control), and column 3 is target siRNA with transfection agent. Densitometric scanning of the products from the 48-h post-transfection samples (column 3, Akt lane) displayed a approximately 85% decrease in the level of Akt demonstrating a successful inhibition of the protein in the primary cultures of gingival epithelial cells.