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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: Cell. 2010 May 28;141(5):775–785. doi: 10.1016/j.cell.2010.03.055

Figure 1. Hoxb8 Cell Lineage Gives Rise to Brain Microglia.

Figure 1

(A-F) Analysis of Hoxb8 lineage in mice heterozygous for the Hoxb8-IRES-Cre and ROSA-YFP alleles. To determine if cells of Hoxb8 lineage in the brain are microglia, the identity of YFP-positive cells was examined by immunohistochemistry. Sagittal sections of the adult cerebral cortex were co-stained with (A) anti-GFP antibody and (B) anti-CD11b antibody. (C) Co-localization of both signals shows that these cells are microglia. (D) Cortical microglia originating from the Hoxb8 cell lineage first appear in the brain during the first two postnatal days (P2), in the choroid plexus and in association with the ventricular lining. (E) The number of YFP-positive cells markedly increases by P14 throughout the cerebral cortex. This high abundance is maintained in the adult life (F). CP, Choroid plexus; CC, cerebral cortex. See supplemental Figure 1.