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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Cancer Res. 2010 May 25;70(11):4385–4393. doi: 10.1158/0008-5472.CAN-10-0070

Figure 2.

Figure 2

Both of the 14-3-3 binding motifs in Snail are required for optimal interaction with 14-3-3 proteins. (A) The truncated forms of Snail used. (B) Interaction of 14-3-3 proteins with truncated Snail variants. The “*” indicates a non-specific band. (C) Single amino acid substitutions in the 14-3-3 binding motifs of Snail. The core serine in the NH2-terminal motif and the threonine in the COOH-terminal motif were replaced with alanines, respectively. (D) The SNA-A and SNA-B sites are required for optimal 14-3-3 protein binding.