Skip to main content
. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Cancer Res. 2010 May 25;70(11):4385–4393. doi: 10.1158/0008-5472.CAN-10-0070

Figure 4.

Figure 4

The COOH-terminal motif SNA-B is required for Snail to induce EMT and cell migration in MCF10 cells. (A) Mutation of SNA-B inhibits Snail-medidated repression on endogenous E-cadherin on induction of mesenchymal markers. MCF10A cells were infected with lentiviral vectors encoding the COOH-terminal Flag-tagged Snail wild type, its mutant variants and GFP. Whole cell extracts were prepared from exponentially growing MCF10A cells and western blots were performed using the indicated antibodies. (B) A functional SNA-B motif is essential for Snail to induce morphological changes in MCF10A cells. Images were taken under the phase-contrast microscope. GFP was used for the indicator of the infection efficiency. (C) Cell migration assays. Each condition was performed in triplicate: the average number of cells per field is represented.