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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Cancer Res. 2010 May 25;70(11):4385–4393. doi: 10.1158/0008-5472.CAN-10-0070

Figure 6.

Figure 6

14-3-3 proeins are recruited to the E-cadherin promoter via an interaction with Snail. (A) Depletion of Ajuba in MCF10A cells inhibits Snail-mediated repression on E-cadherin and induction of Fibronectin and Vimentin. (B) Snail does not induce morphological changes in MCF10A-siAjuba cells. (C) PCR analysis of the immunoprecipitated DNA fragments from MCF10A cells. ChIP assays were performed in the MCF10A-Snail and the MCF10A-ΔSNA-AB cells using the indicated antibodies. The PCR primers flanking the Snail binding sites in the proximal promoter produces a 200bp fragment detected by ethidium bromide staining. (D) The cartoon shows a model for how Snail, Ajuba and 14-3-3 may interact with target genes in chromatin.