Figure 10.

Detection of NDCBE in the DCN by indirect immunofluorescence. A, A confocal laser-scanned image showing labeling of NDCBE in the DCN. Putative CWCs are concentrated in the middle row of the image, and two of them are labeled with asterisks. Arrows, UBCs. B, Another DCN section processed at the same time with the one in A, but without the primary antibody. Orientation of the DCN section in both A and B is the ependymal surface toward the bottom. C, A piece of cerebellum attached to the brainstem section containing the DCN of A shows labeling. Purkinje cells are marked with asterisks and one UBC is marked with an arrow. Images of cerebellar sections not treated with the primary antibody were as dark as the one in B (data not shown). Scale bar, 20 μm. NDCBE antibody was the 1G10 clone (1:100). Laser and confocal settings were identical in all three micrographs.